Abstract: We collected eggs and nestling plasma of ospreys, and prey fish samples between 1999 and 2001 from water bodies situated at varying elevations and degrees of past chlorinated hydrocarbon input throughout British Columbia. Osprey eggs were analyzed immediately after collection and nests with elevated DDE egg concentrations targeted for satellite tagging of adults. Blood samples were taken from chicks. A subset of study areas and nests were observed during the breeding season to identify osprey prey species. Satellite tracking (N = 20) revealed wintering locations of ospreys, where we attempted to make further diet observations and collect fish samples. Highest concentrations of DDE (62 mg/kg wet wt.) in fish were found in samples of rainbow trout from a small lake at 1817m elevation in Revelstoke Park. With the exception of an egg from a mid-elevation lake in Alberta, with 12 mg/kg DDE, all osprey eggs had < 5 mg/kg DDE. Plasma sampling of chicks did not indicate any exceptional uptake of DDE in alpine drainages; chicks from the south Okanagan valley, an area of known DDE contamination, exhibited breeding ground uptake of DDE. Sampling of prey fish from both breeding and wintering grounds were consistent with the generally low levels of chlorinated hydrocarbons in osprey samples.